EFFECTS OF DIETARY GARLIC SUPPLEMENTATION ON SPERM RESERVES, SERUM ANTIOXIDANT ENZYMES AND MALONDIALDEHYDE CONCENTRATION OF RABBIT BUCKS

Authors

  • M Shinkut Agricultural Research Council of Nigeria, Plot 223D Cadastral Zone B3, P. M. B. 5026, Mabushi, Abuja. Nigeria.
  • F. U. Samuel Biotechnology Research Programme, National Animal Production Research Institute, Ahmadu Bello University, Zaria. Nigeria.
  • J. S. Bugau Department of Veterinary Physiology, Ahmadu Bello University, Zaria. Nigeria.
  • A. A. James Department of Veterinary Medicine, Surgery and Radiology.Faculty of Veterinary Medicine, University of Jos. Nigeria.
  • P. I. Rekwot Artificial Insemination Unit, National Animal Production Research Institute, Ahmadu Bello University, Zaria. Nigeria.
  • T Aluwong Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria.

Keywords:

Sperm reserves, Serum Antioxidant Enzymes,Malondialdehyde, Rabbit bucks, Allium sativum

Abstract

The aim of the study was to evaluate the effects of crude Allium sativum(garlic)in diets of rabbit bucks on sperm reserves, serum superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) concentration. Twenty-one bucks were used for the study and were randomly divided into 3 groups of 7 bucks each. Bucks in Group A served as control, while bucks in groups B and C were fed diets containing 2.5% and 5.0 % garlic respectively. The study lasted for 63 days after the period of acclimatization, Blood samples were collected on the last day of the study into collecting tubes without anticoagulant for serum antioxidant enzymes assay. Six bucks from each group were sacrificed and the testes and epididymis were harvested for sperm reserves determination. There were significant (P<0.05) increase in serum superoxide dismutase, catalase and glutathione peroxidase activities and a decrease in MDA concentration in serum of treated animals compared to bucks in the control. There were also significant (P<0.05) increase in sperm reserve of the left testis with increase in garlic dose. Both left and right cauda sperm reserves were significantly (P< 0.05) higher than the control in a dose dependent fashion. Testicular and epididymal reserves, showed increased number of sperm cells in the left testicles and epididymis than the right. Sperm reserve correlated positively with antioxidant enzymes and negatively with malondialdehyde concentration. From the result of the study it can be concluded that adding Allium sativum at 2.5% and 5.0 % in diets of rabbit bucks enhances serum antioxidant enzymes concentration, decreases MDA levels and improves sperm reserves concentration.

Published

2020-06-30